How to Prepare HEPES-Buffered Saline (2X HBS)
2X HBS for calcium phosphate transfection
Components
| Chemical | Concentration |
|---|---|
| HEPES | 50 mM (pH 7.05) |
| NaCl | 280 mM |
| Na2HPO4 | 1.5 mM |
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Ringer's Solution
Isotonic physiological solution for tissue and organ perfusion
Krebs-Ringer Bicarbonate Buffer
Physiological buffer for tissue perfusion and in vitro organ studies, requires 5% CO2
HBSS (Hank's Balanced Salt Solution)
Balanced salt solution for cell washing, tissue transport, and dilution of cells
HEPES-Buffered Saline (HBS)
2X HBS for calcium phosphate transfection of mammalian cells
Normal Saline (0.9% NaCl)
Isotonic saline solution (154 mM NaCl), physiological osmolarity