How to Prepare HEPES-Buffered Saline (HBS)

2X HBS for calcium phosphate transfection of mammalian cells

Chemical	Concentration	Role	MW
HEPES	50 mM (pH 7.05)	buffer	238.3 g/mol
NaCl	280 mM	ionic strength	58.44 g/mol
Na2HPO4	1.5 mM	buffer	141.96 g/mol

Need a different volume or concentration?

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