How to Prepare Krebs-Ringer Bicarbonate Buffer

Physiological buffer for tissue perfusion and in vitro organ studies, requires 5% CO2

Components

Chemical	Concentration	Role	MW
NaCl	119 mM	osmolarity	58.44 g/mol
KCl	4.7 mM	ionic	74.55 g/mol
CaCl2	2.5 mM	divalent cation	110.98 g/mol
MgSO4	1.2 mM	divalent cation	120.37 g/mol
KH2PO4	1.2 mM	buffer	136.09 g/mol
NaHCO3	25 mM	buffer	84.01 g/mol
Glucose	11 mM	energy source	180.16 g/mol

Need a different volume or concentration?

Customize this solution

Related Solutions

Ringer's Solution

Isotonic physiological solution for tissue and organ perfusion

HBSS (Hank's Balanced Salt Solution)

Balanced salt solution for cell washing, tissue transport, and dilution of cells

HEPES-Buffered Saline (HBS)

2X HBS for calcium phosphate transfection of mammalian cells

Normal Saline (0.9% NaCl)

Isotonic saline solution (154 mM NaCl), physiological osmolarity