How to Prepare HBSS (Hank's Balanced Salt Solution)

Balanced salt solution for cell washing and tissue transport

Components

Chemical	Concentration	Role	MW
NaCl	137 mM	osmolarity	58.44 g/mol
KCl	5.4 mM	ionic	74.55 g/mol
Na2HPO4	0.34 mM	buffer	141.96 g/mol
KH2PO4	0.44 mM	buffer	136.09 g/mol
CaCl2	1.3 mM	divalent cation	110.98 g/mol
MgSO4	0.8 mM	divalent cation	120.37 g/mol
NaHCO3	4.2 mM	buffer	84.01 g/mol
Glucose	5.6 mM	energy source	180.16 g/mol

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