How to Prepare 50 mM Tris-HCl Buffer (pH 7.4)

General purpose Tris buffer at physiological pH, adjust with HCl

Components

ChemicalConcentration
Tris base50 mM (pH 7.4)

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Related Solutions

1X PBS (Phosphate Buffered Saline)

Standard physiological buffer pH 7.4, isotonic for mammalian cells

10X PBS Stock

10X concentrated PBS stock solution, dilute 1:10 for working solution

DPBS (Dulbecco's Phosphate Buffered Saline)

PBS with calcium and magnesium for cell culture applications

PBST (PBS-Tween 20)

PBS with 0.1% Tween 20, standard wash buffer for ELISA and Western blot

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1M Tris-HCl stock buffer pH 8.0 for molecular biology applications

TBS (Tris-Buffered Saline)

Tris-buffered saline for immunohistochemistry and Western blot