How to Prepare 10x PBS Stock Solution
10X concentrated PBS stock solution, dilute 1:10 for working solution
Components
| Chemical | Concentration |
|---|---|
| NaCl | 1.37 M |
| KCl | 27 mM |
| Na2HPO4 | 100 mM |
| KH2PO4 | 18 mM |
Preparation Strategy
Method: direct_dissolution
Reason: All component masses are ≥1g, allowing direct dissolution approach
Preparation Table
| Component | Conc. | Amount | Unit |
|---|---|---|---|
| NaCl | 1.37 M | 8.01 | g |
| KCl | 27 mM | 0.20 | g |
| Na2HPO4 | 100 mM | 1.42 | g |
| KH2PO4 | 18 mM | 0.24 | g |
Procedure
- 1Add approximately 80 mL of distilled water to a 100 mL volumetric flask or beaker
- 2Add 8.01 g NaCl and stir until completely dissolved
- 3Add 0.20 g KCl slowly while stirring to prevent localized supersaturation
- 4Add 1.42 g Na2HPO4 and stir until dissolved
- 5Add 0.24 g KH2PO4 and stir until dissolved
- 6Adjust volume to exactly 100 mL with distilled water
- 7Mix thoroughly by inversion or stirring
- 8Verify solution is crystal clear before use
Sterilization
Autoclave at 121°C, 15 psi for 15 minutes. Allow to cool before use. Solution is stable during autoclaving.
Safety
Level 1 safety: Standard laboratory gloves, lab coat, and safety glasses. All components are non-hazardous salts.
Storage
Store at room temperature in tightly sealed container. Stable for 1 year. If crystallization occurs during cold storage, warm to room temperature and redissolve before use.
Source
Cold Spring Harbor Protocols
Phosphate-Buffered Saline (PBS) (Cold Spring Harbor Protocols)
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