How to Prepare TBS (Tris-Buffered Saline)
Tris-buffered saline for immunohistochemistry and Western blot
Components
| Chemical | Concentration |
|---|---|
| Tris base | 20 mM (pH 7.6) |
| NaCl | 150 mM |
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1X PBS (Phosphate Buffered Saline)
Standard physiological buffer pH 7.4, isotonic for mammalian cells
10X PBS Stock
10X concentrated PBS stock solution, dilute 1:10 for working solution
DPBS (Dulbecco's Phosphate Buffered Saline)
PBS with calcium and magnesium for cell culture applications
PBST (PBS-Tween 20)
PBS with 0.1% Tween 20, standard wash buffer for ELISA and Western blot
Tris-HCl Buffer pH 7.4
General purpose Tris buffer at physiological pH, adjust with HCl
Tris-HCl Buffer pH 8.0
1M Tris-HCl stock buffer pH 8.0 for molecular biology applications