How to Prepare Ponceau S Staining Solution

Reversible protein stain for PVDF/nitrocellulose membranes

Components

ChemicalConcentration
Ponceau S0.1% w/v
Acetic acid5% v/v

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Related Solutions

Western Blot Blocking Buffer (BSA)

5% BSA in TBST for Western blot membrane blocking, recommended for phospho-protein detection

Western Blot Blocking Buffer (Milk)

5% non-fat milk in TBST for Western blot blocking, do NOT use for phospho-protein detection

Western Blot Stripping Buffer (Mild)

Mild stripping buffer to remove primary/secondary antibodies from Western blot membranes

Western Blot Stripping Buffer (Harsh)

Harsh stripping at 50-60C for difficult-to-remove antibodies, may damage membrane