How to Prepare 5% Milk Blocking Buffer for Western Blot
5% non-fat milk in TBST, do NOT use for phospho-protein detection
Components
| Chemical | Concentration |
|---|---|
| Non-fat dry milk | 5% w/v |
| Tris base | 20 mM (pH 7.6) |
| NaCl | 150 mM |
| Tween 20 | 0.1% v/v |
Need a different volume or concentration?
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