How to Prepare 5% BSA Blocking Buffer for Western Blot
5% BSA in TBST for Western blot membrane blocking
Components
| Chemical | Concentration |
|---|---|
| BSA | 5% w/v |
| Tris base | 20 mM (pH 7.6) |
| NaCl | 150 mM |
| Tween 20 | 0.1% v/v |
Need a different volume or concentration?
Customize this solutionRelated Solutions
Western Blot Blocking Buffer (Milk)
5% non-fat milk in TBST for Western blot blocking, do NOT use for phospho-protein detection
Western Blot Stripping Buffer (Mild)
Mild stripping buffer to remove primary/secondary antibodies from Western blot membranes
Western Blot Stripping Buffer (Harsh)
Harsh stripping at 50-60C for difficult-to-remove antibodies, may damage membrane
Ponceau S Staining Solution
Reversible protein stain for PVDF/nitrocellulose membranes, used to verify transfer efficiency