How to Prepare Hypotonic Lysis Buffer
Hypotonic buffer causes osmotic swelling for gentle cell lysis, used for nuclear extraction
Components
| Chemical | Concentration |
|---|---|
| Tris-HCl | 10 mM (pH 7.5) |
| KCl | 10 mM |
| MgCl2 | 1.5 mM |
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