How to Prepare IP Lysis Buffer (Immunoprecipitation)
Mild lysis buffer designed to preserve protein-protein interactions for IP/co-IP
Components
| Chemical | Concentration |
|---|---|
| Tris-HCl | 25 mM (pH 7.4) |
| NaCl | 150 mM |
| EDTA | 1 mM |
| NP-40 | 1% v/v |
| Glycerol | 5% v/v |
Need a different volume or concentration?
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