How to Prepare NP-40 Lysis Buffer
Mild non-denaturing lysis buffer, preserves protein-protein interactions for co-IP
Components
| Chemical | Concentration |
|---|---|
| Tris-HCl | 50 mM (pH 8) |
| NaCl | 150 mM |
| NP-40 | 1% v/v |
Need a different volume or concentration?
Customize this solutionRelated Solutions
RIPA Buffer
Strong denaturing lysis buffer for total protein extraction, good for Western blot
Triton X-100 Lysis Buffer
Non-denaturing lysis buffer with Triton X-100, suitable for co-IP and enzyme assays
SDS Lysis Buffer
Strong denaturing lysis buffer, fully denatures all proteins
Urea Lysis Buffer
Chaotropic lysis buffer for membrane proteins and inclusion bodies
IP Lysis Buffer (Immunoprecipitation)
Mild lysis buffer designed to preserve protein-protein interactions for IP/co-IP
CHAPS Lysis Buffer
Zwitterionic detergent buffer for membrane protein solubilization preserving native structure