How to Prepare ChIP Wash Buffer (High Salt)

High salt wash buffer for ChIP to remove non-specific chromatin

Components

Chemical	Concentration	Role	MW
Tris-HCl	20 mM (pH 8)	buffer	121.14 g/mol
NaCl	500 mM	stringent wash	58.44 g/mol
EDTA	2 mM	chelator	292.24 g/mol
Triton X-100	1% v/v	detergent	-
SDS	0.1% w/v	detergent	288.38 g/mol

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Related Solutions

ChIP Lysis Buffer

SDS lysis buffer for chromatin immunoprecipitation, followed by sonication

ChIP Dilution Buffer

Dilution buffer to reduce SDS concentration before ChIP antibody incubation

ChIP Wash Buffer (Low Salt)

Low salt wash buffer for ChIP to remove non-specific binding