How to Prepare ChIP Lysis Buffer

SDS lysis buffer for chromatin immunoprecipitation

Components

ChemicalConcentration
Tris-HCl50 mM (pH 8)
EDTA10 mM
SDS1% w/v

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Related Solutions

ChIP Dilution Buffer

Dilution buffer to reduce SDS concentration before ChIP antibody incubation

ChIP Wash Buffer (Low Salt)

Low salt wash buffer for ChIP to remove non-specific binding

ChIP Wash Buffer (High Salt)

High salt wash buffer for ChIP to remove non-specific chromatin