How to Prepare ChIP Dilution Buffer

Dilution buffer to reduce SDS concentration before ChIP antibody incubation

Components

Chemical	Concentration	Role	MW
Tris-HCl	16.7 mM (pH 8)	buffer	121.14 g/mol
NaCl	167 mM	ionic	58.44 g/mol
EDTA	1.2 mM	chelator	292.24 g/mol
Triton X-100	1.1% v/v	detergent	-
SDS	0.01% w/v	detergent	288.38 g/mol

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ChIP Lysis Buffer

SDS lysis buffer for chromatin immunoprecipitation, followed by sonication

ChIP Wash Buffer (Low Salt)

Low salt wash buffer for ChIP to remove non-specific binding

ChIP Wash Buffer (High Salt)

High salt wash buffer for ChIP to remove non-specific chromatin