How to Prepare Alkaline Lysis Solution III (P3)
Neutralization buffer for alkaline lysis miniprep
Components
| Chemical | Concentration |
|---|---|
| Potassium acetate | 3 M (pH 5.5) |
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TE Buffer
Standard DNA/RNA storage buffer
6X DNA Loading Dye
6X concentrated gel loading dye for agarose gel electrophoresis
20X SSC Buffer
Standard hybridization and wash buffer for Southern/Northern blots
Church Hybridization Buffer
Rapid hybridization buffer for Southern/Northern blots
DEPC-Treated Water
RNase-free water for RNA work, autoclave after DEPC treatment to decompose residual DEPC
10X MOPS Running Buffer
10X stock for denaturing RNA agarose gel electrophoresis