How to Prepare 10X MOPS Running Buffer
10X stock for denaturing RNA agarose gel electrophoresis
Components
| Chemical | Concentration |
|---|---|
| MOPS | 200 mM (pH 7) |
| Sodium acetate | 50 mM |
| EDTA | 10 mM (pH 8) |
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DEPC-Treated Water
RNase-free water for RNA work, autoclave after DEPC treatment to decompose residual DEPC
RNA Loading Buffer
Denaturing loading buffer for RNA agarose gels