How to Prepare Church Hybridization Buffer
Rapid hybridization buffer for Southern/Northern blots
Components
| Chemical | Concentration |
|---|---|
| Na2HPO4 | 0.5 M (pH 7.2) |
| SDS | 7% w/v |
| EDTA | 1 mM (pH 8) |
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TE Buffer
Standard DNA/RNA storage buffer
6X DNA Loading Dye
6X concentrated gel loading dye for agarose gel electrophoresis
20X SSC Buffer
Standard hybridization and wash buffer for Southern/Northern blots
DEPC-Treated Water
RNase-free water for RNA work, autoclave after DEPC treatment to decompose residual DEPC
10X MOPS Running Buffer
10X stock for denaturing RNA agarose gel electrophoresis
RNA Loading Buffer
Denaturing loading buffer for RNA agarose gels