How to Prepare SOB Medium
Rich medium for bacterial transformation, SOC without glucose
Components
| Chemical | Concentration |
|---|---|
| Tryptone | 20 g/L |
| Yeast extract | 5 g/L |
| NaCl | 0.5 g/L |
| KCl | 2.5 mM |
| MgCl2 | 10 mM |
| MgSO4 | 10 mM |
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