How to Prepare M9 Minimal Medium

Defined minimal medium for metabolic studies and auxotrophic selection

Chemical	Concentration	Role	MW
Na2HPO4	48 mM	buffer	141.96 g/mol
KH2PO4	22 mM	buffer	136.09 g/mol
NaCl	8.5 mM	ionic	58.44 g/mol
NH4Cl	18.7 mM	nitrogen source	53.49 g/mol
MgSO4	2 mM	cofactor	120.37 g/mol
CaCl2	0.1 mM	cofactor	110.98 g/mol
Glucose	0.4% w/v	carbon source	180.16 g/mol

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