How to Prepare SDS-PAGE Resolving Gel Buffer
4X resolving gel buffer for SDS-PAGE
Components
| Chemical | Concentration |
|---|---|
| Tris-HCl | 1.5 M (pH 8.8) |
| SDS | 0.4% w/v |
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SDS-PAGE Running Buffer (Tris-Glycine-SDS)
Standard SDS-PAGE running buffer (1X), pH ~8.3 unadjusted
10X SDS-PAGE Running Buffer
10X concentrated SDS-PAGE running buffer stock
Western Blot Transfer Buffer (Towbin)
Standard wet transfer buffer for Western blotting, pH ~8.3
2X Laemmli Sample Buffer
2X concentrated SDS-PAGE sample loading buffer with reducing agent
SDS-PAGE Stacking Gel Buffer
4X stacking gel buffer for SDS-PAGE
1X TAE Buffer
Standard agarose gel electrophoresis buffer for DNA, pH ~8.5