How to Prepare FACS Staining Buffer

PBS + 2% FBS + 0.1% sodium azide for flow cytometry

Components

Chemical	Concentration	Role	MW
NaCl	137 mM	isotonic	58.44 g/mol
KCl	2.7 mM	ionic	74.55 g/mol
Na2HPO4	10 mM	buffer	141.96 g/mol
KH2PO4	1.8 mM	buffer	136.09 g/mol
FBS	2% v/v	blocking	-
Sodium azide	0.1% w/v	preservative	65.01 g/mol

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Ammonium-Chloride-Potassium lysis buffer for selective red blood cell lysis