How to Prepare 10% Neutral Buffered Formalin

Standard tissue fixative for histology. Contains ~3.7% formaldehyde. Toxic - use fume hood

Components

Chemical	Concentration	Role	MW
Formaldehyde (37%)	100 mL/L	fixative	-
NaH2PO4	4 g/L	buffer	119.98 g/mol
Na2HPO4	6.5 g/L	buffer	141.96 g/mol

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4% Paraformaldehyde (PFA)

4% PFA in PBS fixative for cells and tissues. Prepare in fume hood - toxic fumes